Solvent dependences of concentration effect in gel permeation chromatography
نویسندگان
چکیده
منابع مشابه
High Performance Gel Permeation Chromatography of Polymers
The determination of the molecular weight distribution by gel permeation chromatography requires the establishment of a molecular weight calibration and efficient columns in order to minimise chromatogram broadening. The separation process is described in terms of steric exclu— sion, retardation and partial exclusion mechanisms in order to show how the universal calibration method may be influe...
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A major problem in gel permeation chromatography (GPC) or size exclusion chromatography is non-specific binding of applied proteins to the column matrix (stationary phase). We have tested an aqueous arginine solution as the GPC mobile phase on silica-based and polymer-based columns, using mouse monoclonal antibody and recombinant human activin, interleukin-6, basic fibroblast growth factor, and...
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Concentration dependence of elution volume was determined for Blue Dextran 2000, Dextran 500, Dextran sulphate 500 and bovine serum albumin on columns of Sephadex G-100 equilibrated with sodium phosphate buffer, I 0.1, pH6.8. From the results for Dextran 500, it was shown that a linear relation exists between elution volume and the corresponding osmotic pressure calculated for the same concentr...
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ژورنال
عنوان ژورنال: Journal of Applied Polymer Science
سال: 1974
ISSN: 0021-8995,1097-4628
DOI: 10.1002/app.1974.070180422